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13.12.2012 Official
Journal
of
the
European
Union L
340/29
COMMISSION REGULATION (EU) No 1190/2012
of 12 December 2012
concerning a Union target for the reduction of Salmonella Enteritidis and Salmonella Typhimurium
in flocks of turkeys, as provided for in Regulation (EC) No 2160/2003 of the European Parliament
and of the Council
(Text with EEA relevance)
THE EUROPEAN COMMISSION,
ments, in particular in the framework of information
provided for in Directive 2003/99/EC of the European
Parliament and of the Council of 17 November 2003 on
Having regard to the Treaty on the Functioning of the European
the monitoring of zoonoses and zoonotic agents,
Union,
amending Council Decision 90/424/EEC and repealing
Council Directive 92/117/EEC ( 3 ), and in particular
Article 5 thereof, is to be taken into account when
Having regard to Regulation (EC) No 2160/2003 of the
setting the Union target.
European Parliament and of the Council of 17 November
2003 on the control of salmonella and other specified food-
borne zoonotic agents
( 1 ) and, in particular the second
(5) Commission
Regulation
(EC)
No
584/2008
of
20
June
subparagraph of Article 4(1),
2008 implementing Regulation (EC) No 2160/2003 of
the European Parliament and of the Council as regards a
Community target for the reduction of the prevalence of
Whereas:
Salmonella Enteritidis and Salmonella Typhimurium in
turkeys ( 4 ) sets the target for the maximum percentage
of flocks of turkeys remaining positive for those two
(1) The
purpose
of
Regulation
(EC)
No
2160/2003
is
to
Salmonella serotypes to 1 % or less by 31 December
ensure that proper and effective measures are taken to
2012, both for fattening and adult breeding flocks of
detect and control Salmonella and other zoonotic agents
turkeys.
at all relevant stages of production, processing and
distribution, particularly at the level of primary
production, in order to reduce their prevalence and the
(6) The
European
Union
Summary
Report
on
Trends
and
risk they pose to public health.
Sources of Zoonoses, Zoonotic Agents and Food-borne
Outbreaks in 2010 ( 5 ) showed that Salmonella Enteritidis
and Salmonella Typhimurium are the serotypes most
(2) Regulation
(EC)
No
2160/2003
provides
for
a
Union
frequently associated with human illness. In particular
target to be established for the reduction of the
human cases caused by Salmonella Enteritidis further
prevalence of all Salmonella serotypes with public health
decreased markedly in 2010.
significance in turkeys at the level of primary production.
That reduction is key to ensuring that the criteria for
Salmonella in fresh meat of turkeys set out in Part E of
(7) In
March
2012,
EFSA
adopted
a
Scientific
Opinion
on
an
Annex II to that Regulation and in Chapter 1 of Annex I
estimation of the public health impact of setting a new
to Commission Regulation (EC) No 2073/2005 of
target for the reduction of Salmonella in turkeys ( 6 ). It
15 November 2005 on microbiological criteria for food
concluded that Salmonella Enteritidis is the most
stuffs ( 2 ) can be met.
successfully transmitted zoonotic Salmonella serotype
from parent to offspring in poultry. EFSA also
observed that Union control measures in turkeys have
(3) Regulation
(EC)
No
2160/2003
provides
that
the
Union
contributed to a considerable reduction in the number
target is to include a numerical expression of the
of turkey-associated human salmonellosis cases compared
maximum percentage of epidemiological units
to the situation in 2007. The target should therefore be
remaining positive and/or the minimum percentage of
confirmed.
reduction in the number of epidemiological units
remaining positive, the maximum time limit within
which the target must be achieved and the definition
(8) Monophasic
strains
of Salmonella Typhimurium have
of the testing schemes necessary to verify achievement
developed to be among the most frequently detected
of the target. It is also to include a definition, where
Salmonella serotypes in several species of animals and
relevant, of serotypes with public health significance.
in clinical isolates from humans in recent years as
pointed out in the European Union Summary Report
on Trends and Sources of Zoonoses, Zoonotic Agents
(4) Regulation
(EC)
No
2160/2003
provides
that
experience
and Food-borne Outbreaks in 2010. EFSA’s 2010
gained under existing national measures and information
Scientific Opinion on monitoring and assessment of
forwarded to the Commission or to the European Food
Safety Authority (‘EFSA’) under existing Union require
( 3 ) OJ L 325, 12.12.2003, p. 31.
( 4 ) OJ L 162, 21.6.2008, p. 3.
( 1 ) OJ L 325, 12.12.2003, p. 1.
( 5 ) EFSA Journal 2012; 10(3):2597.
( 2 ) OJ L 338, 22.12.2005, p. 1.
( 6 ) EFSA Journal 2012; 10(4):2616.

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L 340/30
EN
Official Journal of the European Union
13.12.2012
the public health risk of ‘Salmonella Typhimurium-like
(a) a reduction of the maximum annual percentage of fattening
strains’ adopted on 22 September 2010 ( 1 ) also stated
turkey flocks remaining positive of Salmonella Enteritidis and
that monophasic Salmonella Typhimurium strains with
Salmonella Typhimurium to 1 % or less; and
the antigenic formula 1,4,[5],12:i:-, which includes
strains with and without the O5 antigen, have to be
(b) a reduction of the maximum annual percentage of adult
considered to be variants of Salmonella Typhimurium
breeding turkey flocks remaining positive of Salmonella
and to pose a public health risk comparable to that of
Enteritidis and Salmonella Typhimurium to 1 % or less.
other Salmonella Typhimurium strains. Salmonella Typhi
murium strains with the antigenic formula 1,4,[5],12:i:-
should therefore be included in the target.
However, for Member States with less than 100 flocks of adult
breeding or fattening turkeys, the Union target shall be that
annually no more than one flock of adult breeding or
(9) To
verify
whether
the
Union
target
has
been
met,
it
is
necessary to sample flocks of turkeys repeatedly. To
fattening turkeys may remain positive.
evaluate and compare the results, it is necessary to
describe a common testing scheme.
As regards monophasic Salmonella Typhimurium, serotypes with
the antigenic formula 1,4,[5],12:i:- shall be included in the
(10) National
control
programmes
for
the
achievement
of
the
Union target.
Union target for 2013 for flocks of turkeys have been
submitted for Union co-financing in accordance with
2. The
testing
scheme
necessary
to
verify
progress
in
the
Council Decision 2009/470/EC of 25 May 2009 on
achievement of the Union target is set out in the Annex
expenditure in the veterinary field ( 2 ). The technical
(‘testing scheme’).
amendments introduced in the Annex to this Regulation
are directly applicable. As a result the Commission does
Article 2
not need to re-approve national control programmes
implementing this Regulation. A transitional period is
Review of the Union target
therefore not needed.
The Union target shall be reviewed by the Commission taking
into account the information collected in accordance with the
(11) In
the
interest
of
clarity,
Regulation
(EC)
No
584/2008
testing scheme and the criteria laid down in Article 4(6)(c) of
should be repealed.
Regulation (EC) No 2160/2003.
(12) The
measures
provided
for
in
this
Regulation
are
in
accordance with the opinion of the Standing
Article 3
Committee on the Food Chain and Animal Health and
Repeal of Regulation (EC) No 584/2008
neither the European Parliament nor the Council has
opposed them,
Regulation (EC) No 584/2008 is repealed.
HAS ADOPTED THIS REGULATION:
References to the repealed Regulation shall be construed as
references to this Regulation.
Article 1
Article 4
Union target
Entry into force
1. The
Union
target,
as
referred
to
in
Article
4(1)
of
Regu
lation (EC) No 2160/2003, for the reduction of Salmonella
This Regulation shall enter into force on the twentieth day
Enteritidis and Salmonella Typhimurium in turkeys (‘Union
following that of its publication in the Official Journal of the
target’) shall be:
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 12 December 2012.
For the Commission
The President
José Manuel BARROSO
( 1 ) EFSA Journal 2010; 8(10):1826.
( 2 ) OJ L 155, 18.6.2009, p. 30.

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EN
13.12.2012 Official
Journal
of
the
European
Union L
340/31
ANNEX
Testing scheme necessary to verify the achievement of the Union target as referred to in Article 1(2)
1. SAMPLING
FRAME
The sampling frame shall comprise all flocks of fattening and breeding turkeys within the framework of the
national control programmes provided for in Article 5 of Regulation (EC) No 2160/2003.
2. MONITORING
IN
TURKEYS
2.1. Frequency
of
sampling
(a) Food business operators shall sample all flocks of fattening and breeding turkeys in the following way:
(i) sampling of flocks of fattening and breeding turkeys shall take place within three weeks before slaughter.
The competent authority may authorise sampling in the last six weeks prior to the date of slaughter in
case the turkeys are either kept more than 100 days or fall under organic turkey production according to
Commission Regulation (EC) No 889/2008 ( 1 );
(ii) sampling of flocks of breeding turkeys shall take place:
— in rearing flocks: at day-old, at four weeks of age and two weeks before moving to the laying phase or
laying unit,
— in adult flocks: at least every third week during the laying period at the holding or at the hatchery,
— on the holding in the case of flocks of breeding turkeys laying hatching eggs intended for the trade
within the Union.
(iii) The competent authority may decide to implement one of the options referred to in the second indent of
point (ii) to the whole testing scheme for all flocks. However, sampling of breeding flocks laying hatching
eggs intended for the trade within the Union must take place on the holding.
(iv) By way of derogation from the second indent of point (ii), if the Union target has been achieved for at
least two consecutive calendar years in the whole Member State, sampling at the holding may be extended
to take place every four weeks, at the discretion of the competent authority. However, the competent
authority may decide to keep or revert to a three-week testing interval in the case of detection of the
presence of the relevant Salmonella serotypes in a breeding flock on the holding and/or in any other case
deemed appropriate by the competent authority.
(b) The sampling by the competent authority shall at least provide for:
(i) sampling of flocks of breeding turkeys:
— once a year, all flocks with at least 250 adult breeding turkeys between 30 and 45 weeks of age and all
holdings with elite, great grand parents and grand parent breeding turkeys; the competent authority
may decide that this sampling may also take place at the hatchery; and
— all flocks on holdings in case of detection of Salmonella Enteritidis or Salmonella Typhimurium from
samples taken at the hatchery by food business operators or within the frame of official controls, to
investigate the origin of infection;
(ii) sampling of flocks of fattening turkeys shall be done once a year, at least in one flock on 10 % of the
holdings with at least 500 fattening turkeys;
(iii) sampling may be done on a risk basis and additionally each time the competent authority considers it
necessary;
(iv) a sampling carried out by the competent authority may replace the sampling by the food business
operator as referred to in point (a).
2.2. Sampling
protocol
2.2.1. General instructions for sampling
The competent authority or the food business operator shall ensure that samples are taken by persons trained for
that purpose.
( 1 ) OJ L 250, 18.9.2008, p. 1.

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L 340/32
EN
Official Journal of the European Union
13.12.2012
Sampling of flocks of breeding turkeys shall be done in accordance with point 2.2 of the Annex to Commission
Regulation (EU) No 200/2010 ( 1 ).
For sampling of flocks of fattening turkeys at least two pairs of boot swabs shall be taken per flock. Boot swabs
are put on the boots and the sample is taken by walking around in the poultry house. Swabs from one flock of
turkeys may be pooled into one sample.
Before putting on the boot swabs, their surface shall be moistened by:
(a) the application of maximum recovery diluents (MRD: 0,8 % sodium chloride, 0,1 % peptone in sterile deionised
water); or
(b) the application of sterile water; or
(c) the application of any other diluents approved by the national reference laboratory referred to in Article 11(3)
of Regulation (EC) No 2160/2003; or
(d) being autoclaved in a container together with diluents.
The way to moisten boot swabs shall be to pour the liquid inside before putting them on or to shake them in a
container of diluent.
It shall be ensured that all sections in a house are represented in the sampling in a proportionate way. Each pair of
boot swabs must cover about 50 % of the area of the house.
On completion of sampling, the swabs shall be carefully removed from the boots so as not to dislodge adherent
material. Boot swabs may be inverted to retain material. They shall be placed in a bag or pot and labelled.
The competent authority may decide to increase the minimum number of samples in order to ensure represen
tative sampling on a case-by-case evaluation of epidemiological parameters, such as biosecurity conditions, the
distribution or size of the flock.
If the competent authority approves one pair of boot swabs may be replaced by a dust sample of 100 g collected
from multiple places throughout the house from surfaces with visible presence of dust. As an alternative, one or
several moistened fabric swab(s) of a combined surface of at least 900 cm 2 may be used to gather dust from
multiple surfaces throughout the house. Each swab shall be well coated with dust on both sides.
2.2.2. Specific instructions for certain types of holdings
(a) For free range flocks of turkeys, samples shall only be collected inside the house.
(b) Where access to the houses is not possible due to limited space in flocks with less than 100 turkeys, and it is
therefore not possible to use boot swabs when walking around, they may be replaced by the same kind of
hand fabric swabs that are used for dust, where the swabs are rubbed over surfaces contaminated with fresh
faeces, or if this is not feasible, by other sampling techniques for faeces fit for the intended purpose.
2.2.3. Sampling by the competent authority
The competent authority shall satisfy itself by conducting further tests and/or documentary checks as appropriate
to verify that results are not altered through the presence of antimicrobials or other substances inhibiting the
growth of bacteria.
Where the presence of Salmonella Enteritidis and Salmonella Typhimurium is not detected but antimicrobials or
bacterial growth inhibitory effects are detected, the flock shall be considered to be an infected flock of turkeys for
the purpose of the Union target referred to in Article 1(2).
2.2.4. Transport
Samples shall be sent without undue delay either by via express mail or courier, to the laboratories referred to in
Articles 11 and 12 of Regulation (EC) No 2160/2003. During transport they shall be protected from heat over
25 °C and exposure to sunlight.
Where it is not possible to send the samples within 24 hours from the time of sampling they shall be stored
refrigerated.
3. LABORATORY
ANALYSES
3.1. Preparation
of
the
samples
At the laboratory samples shall be kept refrigerated until examination, which shall be started within 48 hours
following receipt and within 96 hours after sampling.
( 1 ) OJ L 61, 11.3.2010, p. 1.

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EN
13.12.2012 Official
Journal
of
the
European
Union L
340/33
The pair(s) of boot/sock swabs shall be carefully unpacked to avoid dislodging adherent faecal material, pooled and
placed in 225 ml buffered peptone water (BPW) which has been pre-warmed to room temperature. The boot/sock
swabs shall be fully immersed in BPW and therefore more BPW may be added if necessary.
The dust sample shall preferably be analysed separately. However for fattening flocks, the competent authority may
decide to allow it to be pooled with the pair of boot/sock swabs for analysis.
The sample shall be swirled to fully saturate it and culture shall be continued by using the detection method set
out in point 3.2.
Other samples (e.g. from breeding flocks or hatcheries) shall be prepared in accordance with point 2.2.2 of the
Annex to Regulation (EU) No 200/2010.
If standards of European Committee for Standardisation (‘CEN’) or the International Organisation for Standard
isation (‘ISO’) on the preparation of faeces for the detection of Salmonella are agreed on, they shall be applied and
replace the provisions on the preparation of samples set out in this point.
3.2. Detection
method
The detection method recommended by the EU reference laboratory for Salmonella in Bilthoven, the Netherlands,
shall be used.
That method is described in the Annex D to EN/ISO 6579 (2002): ‘Detection of Salmonella spp. in animal faeces
and in samples of the primary production stage’.
In that detection method, a semi-solid medium (modified semi-solid Rappaport-Vassiliadis medium, MSRV) is used
as the single selective enrichment medium.
3.3. Serotyping
For breeding flocks of turkeys, at least one isolate from each positive sample shall be serotyped, following the
White-Kauffmann-Le Minor scheme.
For fattening flocks of turkeys, at least one isolate from each positive sample taken by the competent authority
shall be serotyped, following the White-Kauffmann-Le Minor scheme.
Food business operators shall at least ensure that for all isolates none of them belong to the serotypes Salmonella
Enteritidis or Salmonella Typhimurium including monophasic strains with the antigenic formula 1,4,[5],12:i:-.
3.4. Alternative
methods
With regard to samples taken on the initiative of the food business operator, the methods of analysis provided for
in Article 11 of Regulation (EC) No 882/2004 of the European Parliament and of the Council ( 1 ), may be used
instead of the methods for the preparation of samples, detection method and serotyping provided for in points
3.1, 3.2 and 3.3 of this Annex, if validated in accordance with EN/ISO 16140.
3.5. Storage
of
strains
Laboratories shall ensure that at least one isolated strain of Salmonella spp. per flock and per year can be collected
by the competent authority and stored for possible future phage typing or anti-microbial susceptibility testing,
using the normal methods for culture collection, which must ensure integrity of the strains for a minimum of two
years from the date of analysis.
The competent authority may decide that isolates of Salmonella spp. from sampling by food business operators
shall also be stored for future phagetyping or antimicrobial susceptibility testing to provide for isolates to be tested
in accordance with Article 2 of Commission Decision 2007/407/EC ( 2 ).
4. RESULTS
AND
REPORTING
4.1. Calculation of prevalence for the verification of the Union target
A flock of turkeys shall be considered positive for the purpose of verifying the achievement of the Union target,
where the presence of Salmonella Enteritidis and/or Salmonella Typhimurium (other than vaccine strains, but
including monophasic strains with the antigenic formula 1,4,[5],12:i:-) was detected in the flock.
( 1 ) OJ L 165, 30.4.2004, p. 1.
( 2 ) OJ L 153, 14.6.2007, p. 26.

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L 340/34
EN
Official Journal of the European Union
13.12.2012
Positive flocks of turkeys shall be counted only once per round, irrespective of the number of sampling and testing
operations and only be reported in the year of the first positive sampling. The prevalence shall be calculated
separately for flocks of fattening turkeys and flocks of adult breeding turkeys.
4.2. Reporting
4.2.1. Reporting shall include:
(a) the total number of flocks of fattening and adult breeding turkeys which were tested at least once during the
year of reporting;
(b) the total number of fattening and adult breeding flocks positive with any Salmonella serotype in the Member
State;
(c) the number of flocks of fattening and adult breeding turkeys tested positive at least once for Salmonella
Enteritidis and Salmonella Typhimurium including monophasic strains with the antigenic formula
1,4,[5],12:i:-;
(d) the number of fattening and adult breeding turkey flocks positive for each Salmonella serotype or for Salmonella
unspecified (isolates that are untypable or not serotyped).
4.2.2. The information referred to in (a) to (d) of point 4.2.1 shall be provided separately for the sampling within the overall national
Salmonella control programme for:
(a) the food business operators sampling as provided for in point 2.1(a); and
(b) the competent authorities sampling as provided for in point 2.1(b).
4.2.3. The results of the tests shall be considered relevant food chain information as provided for in Section III of Annex II to
Regulation (EC) No 853/2004 of the European Parliament and of the Council ( 1 ).
At least the following information shall be made available to the competent authority from each flock of turkeys
tested:
(a) holding reference, remaining unique in time;
(b) flock reference, remaining unique in time;
(c) month of sampling;
(d) number of birds per flocks.
The results and any additional relevant information shall be reported as part of the report on trends and sources
provided for in Article 9(1) of Directive 2003/99/EC of the European Parliament and of the Council ( 2 ).
The food business operator shall notify the competent authority of the detection of Salmonella Enteritidis and
Salmonella Typhimurium including monophasic strains with the antigenic formula 1,4,[5],12:i:- without undue
delay. The food business operator shall instruct the analysing laboratory to act accordingly.
( 1 ) OJ L 139, 30.4.2004, p. 55.
( 2 ) OJ L 325, 12.12.2003, p. 31.

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